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为了得到高维生素C(Vc)含量新品种,克隆和构建了GalUR半乳糖醛酸还原酶基因,并转入了番茄.通过不同转基因突变体GalUR基因表达和Vc含量分析发现:GalUR基因在转GalUR基因番茄中得到了过量表达;在转FRO2-铁还原酶基因的番茄突变体中表达量增加了60%;在耐贮藏转基因番茄F3、F2、F1中也均高于对照,其中F3的表达量最高,是对照的2~3倍;在非转基因中蔬4号和6号的表达量高于丽春番茄1倍;所有供试Ga-lUR基因表达量均为红果最多、粉红果次之,青果最少.转基因番茄果实Vc含量变化与上述GalUR基因的表达有相同规律.转GalUR3基因番茄果实和叶片Vc含量高于对照2倍以上.GalUR基因的表达还与乙烯合成酶基因ACS、CTR1和铁还原酶FRO2基因的表达有关.
In order to obtain a new high vitamin C (Vc) content variety, GalUR galacturonase reductase gene was cloned and transferred into tomato.Analysis of GalUR gene expression and Vc content by different transgenic mutants showed that the GalUR gene was transferred into GalUR Over-expression in transgenic tomato; expression in tomato mutant transformed with FRO2-iron reductase increased by 60%; also in F3, F2 and F1 resistant transgenic tomato, the expression level of F3 The highest was 2 to 3 times that of the control; the expression level of vegetables No.4 and No.6 in non-transgenic lines was 1 times higher than that of Laichun Tomato; the expression levels of Ga-lUR gene in all tested cultivars were the highest in red fruits and the second in pink fruits, The least change in fruit Vc content in transgenic tomato fruits was the same as the expression of GalUR gene mentioned above.The content of Vc in fruits and leaves of transgenic GURUR3 gene was more than 2 times higher than that in control.The expression of Galur gene was also associated with the expression of ethylene synthase genes ACS, CTR1 and iron Reductase FRO2 gene expression.