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目的 探讨赖氨酸、脯氨酸和抗坏血酸钙对去卵巢大鼠骨质疏松的预防作用。 方法 6～ 9月龄雌性大鼠 90只 ,随机分 9组 :盐酸赖氨酸低剂量组 (LL)和高剂量组 (LH) ,脯氨酸低剂量组 (PL)和高剂量组 (PH) ,抗坏血酸钙低剂量组 (CL)和高剂量组 (CH) ,葡萄糖酸钙阳性对照组 (CG)和骨质疏松模型组 (MOD)及假手术组 (SHAM)。术后 4、8、12周取血测酸性磷酸酶 (ACP)、碱性磷酸酶 (ALP)、钙 (Ca)、磷 (P)。 结果 与SHAM和MOD组比较 ,LL组术后体重增加最多 ;LL和LH组血ACP活性〔(2 2 5 0± 1 2 97)U/L〕和ALP活性〔(30 0 3± 136 9)U/L〕增高 ,P <0 0 1;CL组血ALP活性〔(2 94 0± 12 2 2 )U/L〕增高 ,P <0 0 5 ;CH组血Ca〔(2 6 76± 0 0 73)U/L〕升高 ,与SHAM组比较差异有显著性 (P <0 0 5或P <0 0 1)。 结论 赖氨酸和抗坏血酸钙可能对去卵巢大鼠骨质疏松有一定预防作用。
Objective To investigate the preventive effects of lysine, proline and calcium ascorbate on osteoporosis in ovariectomized rats. Methods Ninety-nine female rats aged 6 to 9 months were randomly divided into 9 groups: low dose of lysine hydrochloride (LL) and high dose (LH), low dose of proline (PL) and high dose ), Low dose of calcium ascorbate (CL) and high dose (CH), calcium gluconate positive control group (CG) and osteoporosis model group (MOD) and sham operation group (SHAM). Blood samples were taken for determination of acid phosphatase (ACP), alkaline phosphatase (ALP), calcium (Ca) and phosphorus (P) at 4, 8 and 12 weeks after operation. Results Compared with SHAM group and MOD group, the weight gain of LL group was the highest after operation. The blood ACP activity [(2 2 5 0 ± 1 2 97) U / L〕 and ALP activity 〔(30 0 ± 136 9) U / L] increased, P <0.01; the ALP activity in blood of CL group increased (2 94 0 ± 12 2 2 U / L), P 0 05; 0 73) U / L〕 increased, compared with the SHAM group, the difference was significant (P 0 05 or P 0 01). Conclusion Lysine and calcium ascorbate may prevent osteoporosis in ovariectomized rats.