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为探讨北方地区病毒性脑炎及脑膜炎的病原学构成,采用微量细胞培养法,应用人胚肺二倍体细胞(HEF)、人表皮样癌细胞(Hep-2)、猴肾传代细胞(Vero)及狗肾传代细胞(MDCK)等4种细胞系对86例脑炎、脑膜炎患儿的脑脊液、粪便及咽拭标本进行了病毒分离。结果36例分离到了病毒,阳性率为41.86％(36/86例),其中肠道病毒18例,腺病毒3型(AdV_3)8例,单纯疱疹病毒1型(HSV_1)7例,阳性率分别为20.93％、9.30％、8.14％。此外,还分离到流感病毒(Flu)2例,巨细胞病毒(CMV)1例。证实小儿病毒性脑炎及脑膜炎病原以肠道病毒为主,腺病毒及单纯疱疹病毒次之。
In order to explore the etiological composition of viral encephalitis and meningitis in northern China, the cell culture method was used to detect the etiological characteristics of viral encephalitis and meningitis in North China. HEF, Hep-2, Vero and MDCK were isolated from 86 cases of cerebrospinal fluid, stool and pharyngeal swab in 86 children with encephalitis and meningitis. Results The positive rate of 36 cases was 41.86% (36/86 cases). Among them, 18 cases were enterovirus, 8 cases were adenovirus type 3 (AdV_3) and 7 cases were HSV_1, the positive rate was 20.93%, 9.30%, 8.14%. In addition, two cases of influenza virus (Flu) and one case of cytomegalovirus (CMV) were also isolated. Confirmed that children with viral encephalitis and meningitis pathogens to enterovirus-based, followed by adenovirus and herpes simplex virus.