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Novel LMW-GS(low molecular weight glutenin subunit),α-and γ-gliadin from Triticum macha accessions were characterized via genomic PCR,which can do favor to improve the wheat quality.The complete coding regions of two α-gliadin,two γ-gliadin and two LMW-GS gene sequences,which designed as Gli-Mal,Gli-Ma2,Gli-Mr1,Gli-Mr2,Glu-LM1 and Glu-LM2,encoded the mature proteins with 307,241,348,302,474 and 377 amino acid residues,respectively.Gli-Mal and Gli-Ma2 were recognized as pseudogenes due to the in-frame stop codons.The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α-or γ-gliadin or LMW-m type proteins with the exception of Gli-Ma2.Phylogenetic analysis showed Gli-Mal was closely related to those from T.aestivum,whereas Gli-Ma2 seemed to be more homologous with the gene sequences from Dasypyrum breviaristatum.Gli-Mr1 was closely related to those from T.turgidum ssp.dicoccoides,while Gli-Mr2 was the nearest to those from T.aestivum.Glu-LM1 was closely related to those from Aegilops tauschii,whereas Glu-LM2 seemed to be more homologous with those from T.durum.