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目的 :观察钙通道阻滞剂尼卡地平对低密度脂蛋白 (LDL)体外氧化以及对小鼠腹腔巨噬细胞泡沫化过程中细胞内脂质代谢两个环节的影响 ,探讨钙通道阻滞剂抗动脉粥样硬化 (AS)的作用机制。方法 :采用Cu2 + 氧化LDL ,动态监测LDL氧化修饰过程中 2 34nm处紫外吸光度及丙二醛 (MDA)含量的变化 ,观察不同浓度尼卡地平 (10 μmol/L、10 0 μmol/L)对体外LDL氧化修饰的抑制作用。收集和培养纯系 6 15小鼠腹腔巨噬细胞 ,将其分成 4组 :①对照组 ,②氧化修饰LDL(ox LDL)组 ,③ox LDL加 10 μmol/L尼卡地平组 ,④ox LDL加 10 0μmol/L尼卡地平组 ,培养 4 8h后 ,分别测定细胞内总胆固醇 (TC)、游离胆固醇 (FC)及胆固醇酯 (CE) ,观察不同浓度尼卡地平对小鼠腹腔巨噬细胞泡沫化过程中细胞内脂质代谢的影响。结果 :①尼卡地平可以降低LDL氧化修饰过程中 2 34nm吸光度峰值 ,明显延长迟滞期 ,使氧化曲线右移 ,且存在剂量效应关系。 10 μmol/L及 10 0μmol/L尼卡地平分别将迟滞期延长33.96 %和 6 9.6 5 % ,与对照组比较差异均有显著性意义 (均P <0 .0 5 )。同时 ,尼卡地平可以减少MDA的生成量 ,10 0 μmol/L尼卡地平抑制MDA生成量更明显 ,MDA减少 2 1%。②ox LDL组细胞内TC、FC及CE较对照组均显著增高 (均P <0 .0 1) ,巨噬?
Objective: To observe the effects of nicardipine, a calcium channel blocker, on the in vitro oxidation of low density lipoprotein (LDL) and the intracellular lipid metabolism during the foaming of mouse peritoneal macrophages, and to explore the effects of calcium channel blockers Anti-atherosclerosis (AS) mechanism of action. Methods: The changes of UV absorbance at 2 34nm and the content of malondialdehyde (MDA) were monitored by Cu2 + oxidation of LDL. The effects of different concentrations of nicardipine (10 μmol / L, 100 μmol / L) In vitro inhibition of LDL oxidative modification. The peritoneal macrophages of 615 mice were collected and cultured and divided into 4 groups: ① control group, ② ox LDL group, ③ox LDL plus 10 μmol / L nicardipine group, ④oxLDL plus 10 After 48 h incubation, the total cholesterol (TC), free cholesterol (FC) and cholesteryl ester (CE) were measured in the nicardipine group at 0μmol / L, and the inhibitory effects of nicardipine on the foaming of mouse peritoneal macrophages The influence of intracellular lipid metabolism during the process. Results: (1) Nicardipine can reduce the peak of 2 34 nm absorbance during the oxidative modification of LDL, prolong the lag phase obviously and shift the oxidation curve to the right, and there is a dose-response relationship. The 10 micromol / L and 100 micromol / L nicardipine prolonged the lag phase by 33.96% and 6 9.65%, respectively, which was significantly different from the control group (all P <0.05). At the same time, nicardipine can reduce the production of MDA, nicardipine 100 micromol / L inhibit the formation of MDA more obvious, MDA decreased 21%. ② The levels of TC, FC and CE inoxLDL group were significantly higher than those in control group (all P <0.01)