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利用电镜—细胞化学技术研究了盐胁迫下芦荟(AloeveraL.)叶同化组织细胞中ATP酶(ATPase)分布特性及外源硅(Si)的作用。结果表明,正常生长情况下,芦荟叶同化组织细胞中液泡膜ATPase活性明显强于质膜ATPase,在细胞壁初生纹孔场观察到很强的ATPase活性。NaCl100mmol·L-1处理30d,浇灌的营养液中不加可溶性Si,芦荟叶同化组织细胞中液泡膜、质膜和初生纹孔场ATPase活性显著减弱,浇灌的营养液中加Si2.0mmol·L-1处理,芦荟叶同化组织细胞中ATPase活性则显著增强,尤其是在液泡膜和初生纹孔场。叶同化组织细胞中ATPase活性的差异性反映芦荟叶生理状态和功能的差异性,可溶性Si增强盐胁迫下芦荟ATPase活性是Si缓解芦荟盐胁迫伤害效应的重要细胞生理机制。
The distribution of ATPase and the effects of exogenous silicon (Si) in the leaves of Aloe vera L. alluvial cells under salt stress were studied by electron microscopy-cytochemistry. The results showed that under normal growth conditions, tonoplast ATPase activity in aloe cells was significantly higher than that of plasma membrane ATPase, and strong ATPase activity was observed in the primary cell wall of cell wall. After treatment with100mmol·L-1 NaCl for30days, without adding soluble Si, the ATPase activity of tonoplast, plasma membrane and primary pits in Aloe barbadensis leaves cells was significantly weakened. The nutrient solution supplemented with Si2.0mmol·L -1 treatment, aloe vera leaf tissue ATPase activity was significantly enhanced, especially in the tonoplast and primary pits. The difference of ATPase activity in the cells of the leaf assimilation cells reflected the difference of physiological status and function of Aloe vera leaves. Soluble Si enhances the ATPase activity of Aloe vera aloe under salt stress, which is an important cell physiological mechanism of Si to alleviate the damage effect of Aloe barley salt stress.