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以尾叶桉、窿缘桉、雷林一号桉、赤桉胚性愈伤分离的原生质体为材料，进行了青枯病病原菌提取物对桉树原生质体生长发育影响研究。结果表明，青枯病病原菌提取物对桉树原生质体生长发育的影响主要表现：不同生长时期的青枯菌菌液中，处于对数生长期的提取液对植物的致毒力最强。植物对青枯菌抗性的高低与菌液的加入时间有关，加入时间不宜过早，以原生质处于再生壁后并发育为细胞时加入最为合适。不同桉树品种细胞悬浮液进行抗青枯菌筛选的菌液选择浓度有较大差别，尾叶桉为２０％ 、窿缘桉１５％ 、赤桉１５％ 、雷林一号桉１０％ 。原生质分离材料在加青枯菌提取液的培养基上短期培养有助于原生质及其发育的细胞增强对青枯病的抗性。
Eucalyptus urophylla, Eucalyptus urophylla, Eucalyptus eucalyptus, Eucalyptus embryogenic callus protoplasts were used as material to study the effects of bacterial wilt pathogens on the growth of Eucalyptus protoplasts. The results showed that the effects of bacterial wilt pathogens on the growth and development of Eucalyptus protoplasts were mainly manifested as follows: In the different growth stages of R. solanacearum, the extract from logarithmic growth phase had the strongest virulence to plants. The level of plant resistance to R. solanacearum is related to the adding time of the bacterial liquid, and the adding time should not be too early. When the protoplast is in the regenerating wall and is developed into the cell, it is most suitable. Eucalyptus varieties of cell suspension for selection of bacteria resistant to R. solanacearum selection concentration is quite different, 20% of Eucalyptus urophylla, eucalyptus 15%, Eucalyptus 15%, Eucalyptus 10%. Short-term culture of protoplast isolation material on medium supplemented with R. solanacearum helps protoplasts and their developing cells enhance resistance to bacterial wilt.