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利用16S rDNA-RFLP和全序列测定方法,对分离自商洛地区5个分布点的59株多花胡枝子根瘤菌进行了RFLP分析和系统发育研究.结果表明:(1)42株供试菌株归属根瘤菌属(Rhizobium)、11株归属中华根瘤菌属(Sinorhizobium).其余6株非根瘤菌中3株是嗜麦芽黄单胞菌(Stenotrophomonas maltophilia)、3株是解淀粉类芽孢杆菌(Paenibacillus amylolyticus),说明胡枝子根瘤内生菌较为丰富且类型多样.(2)结合供试菌株的地理生境分析,发现来自不同采集点的菌株有些具有同样的遗传类型,而来自同一采集点的菌株遗传类型却有差异,证明胡枝子根瘤菌在分群类别上与地理环境之间没有明确的对应关系,地理环境并非根瘤菌多样性形成的主要因素.建议今后对根瘤菌多样性研究应从根瘤菌与寄主植物物之间的共生选择进化,特别是对共生体系中基因的横向转移方面进行深入探讨.
The results of RFLP analysis and phylogenetic analysis of 59 Rhizobium multiflora strains isolated from five distribution points in Shangluo region by 16S rDNA-RFLP and full sequence assay showed that: (1) The 42 strains tested belonged 11 belonged to Sinorhizobium, 3 belonged to Stenotrophomonas maltophilia and 3 strains belonged to Paenibacillus amylolyticus (Rhizobium) ), Indicating that Rhizobia endodermis endophyte more abundant and diverse types. (2) Combined with the analysis of the geographical isolates of the test strains, strains from different collection points found that some of the same genetic types, and from the same collection point strain genetic type There is a difference between the Rhizobium strains of rhizobium and host plants, indicating that rhizobia does not have a clear correspondence with the geographical environment and the geographical environment is not the main factor for the formation of rhizobia diversity. The symbiotic evolution between the two species evolves, especially in terms of the horizontal transfer of genes in the symbiotic system.