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研究寡肽蛋-苏-精-缬(MTRV)抑制多聚次黄苷酸-胞苷酸(poly I∶C)诱发的小鼠胚胎吸收的免疫机制。给予异基因妊娠BALB/c×C57BL/6小鼠腹腔注射poly I∶C建立诱发性胚胎吸收模型;在孕期多个时点腹腔注射MTRV,观察其对胚胎吸收率的影响,并采用流式细胞术检测妊娠9.5 d和13.5 d妊娠子宫内各种免疫活性细胞的百分率。结果显示,MTRV不能显著改变poly I∶C所引起的Toll样受体3阳性细胞和CD80阳性细胞增多,但是可显著抑制poly I∶C所引起的IFN-γ阳性细胞增多和转化生长因子-β阳性细胞减少,并降低流产率。提示寡肽MTRV可纠正poly I∶C所引起的妊娠子宫内细胞因子表达紊乱,从而抑制poly I∶C所诱发的流产。
To investigate the immunological mechanism of the oligopeptide E-suetrin-valine (MTRV) in inhibiting poly I: C-induced mouse embryo resorption. Allogeneic pregnancy BALB / c × C57BL / 6 mice were injected intraperitoneally with poly I: C to establish induced embryo absorption model. MTRV was injected intraperitoneally at various time points during pregnancy to observe its effect on embryo absorption. Flow cytometry The percentages of various intrauterine immunocompetent cells on gestational 9.5 d and 13.5 d pregnancies were determined. The results showed that MTRV could not significantly change poly I: C induced Toll-like receptor 3-positive cells and CD80-positive cells increased, but could significantly inhibit poly I: C-induced IFN-γ-positive cells and transforming growth factor-β Reduced positive cells and reduced abortion rates. Prompt oligopeptide MTRV can correct poly I: C caused by intrauterine pregnancy cytokine expression disorder, thereby inhibiting poly I: C-induced abortion.