论文部分内容阅读
从白车轴草 (Trifoliumrepens)自然种群中采集无白车轴草单孢锈菌病史的无性系 (clones) 17个 ,有白车轴草单孢锈菌病史的无性系 14个 ,分别作为抗病型和感受型植物实验材料 ;采集白车轴草单孢锈菌 (Uromycestrifolii repentis)菌系 (strains) 10个 ,作为病菌实验材料 .分别设置并进行了两个温室实验、一个田间盆栽实验和一个原生长地移栽实验 ,实验处理上分对照、单菌系接种和 10个菌系接种等 3种 .实验结果表明 ,无论是用单菌系接种还是 10个菌系接种 ,植株发病的概率和程度均与其抗病性有关 ,抗病型植株 (无病史 )发病的概率和程度显著低于感受型 (有病史 )植株 .在相同处理的实验中 (无论是田间实验还是温室实验 ) ,无病史植株和有病史植株的生长无显著差异 ;不同处理田间实验植株的生长有显著差异 ,病情愈重 ,生长愈差 .无病史植株的抗病性明显强于有病史植株 .但是 ,原生长地的移栽实验结果表明 ,在无病原菌存在的情况下 ,有病史植株的 (叶 )生长显著好于无病史植株 .可以认为 ,研究生物个体对环境因子反应性差异的实验应当在自然条件下和自然梯度范围内进行 .
Seventeen clones were collected from the natural population of Trifolium repens without any history of single-spore rust, and 14 clones with a history of single-spore rust of Caterpillar fungus were used as anti-disease and anti- 10 experimental plants of Uromycestrifolii repentis were collected as experimental materials for the pathogenic bacteria, and two greenhouse experiments, one field pot experiment and one original growth stage Transplanting experiments, experimental treatment on the sub-control, single bacteria inoculation and 10 strains of inoculation, etc. The experimental results show that no matter with single bacteria inoculation or 10 strains inoculation, the probability and extent of the incidence of plants are Disease-resistant plants, the probability and degree of disease-resistant plants (disease-free history) were significantly lower than that of the susceptible (disease-history) plants.In the same treatment experiment (whether field experiment or greenhouse experiment) There was no significant difference in the growth of the history of the plants; the growth of the experimental plants in different treatment fields were significantly different, the more serious the disease, the worse the growth.Without the history of the disease resistance of plants was significantly stronger than the history of plants However, the transplanting experiments in native areas showed that in the absence of pathogenic bacteria, the growth of plants with a history of disease was significantly better than that of the disease-free history. It can be considered that the experiment to study the differences in the reactivity of environmental organisms among biological individuals It should be carried out under natural conditions and within the natural gradient.