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目的建立HPLC双波长法同时测定药桑中芦丁、异虎皮苷、槲皮素和绿原酸含量的方法。方法采用Shim-pack VP-ODS(250mm×4.6mm,5μm)色谱柱;流动相A:乙腈,流动相B:20mL.L-1冰醋酸和55mL.L-1乙腈混合水溶液,pH2.55,梯度洗脱;检测波长335和266nm;流速1.0mL.min-1;柱温37℃。结果芦丁、异虎皮苷、槲皮素和绿原酸分别在5.05～80.15μg.mL-1(r=0.999 1),5.00～80μg.mL-1(r=0.999 7),0.53～8.12μg.mL-1(r=0.999 2),5.30～80.12μg.mL-1(r=0.999 2)范围内与峰面积呈良好的线性关系。方法的加样回收率(n=9)分别为100.5%,101.6%,103.4%和101.8%;RSD分别为4.8%,7.4%,2.7%和6.3%。结论该法快速、简便、准确、重复性好,适用于药桑的质量控制。
OBJECTIVE To establish a method for the simultaneous determination of rutin, iso-cerebral glycoside, quercetin and chlorogenic acid in medicinal mulberry by HPLC dual-wavelength method. Methods A Shim-pack VP-ODS (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase A was acetonitrile, the mobile phase B was 20 mL, the mixed aqueous solution of L-1 glacial acetic acid and 55 mL L- Gradient elution; detection wavelength 335 and 266nm; flow rate 1.0mL.min-1; column temperature 37 ℃. Results Rutin, isoproterenol, quercetin and chlorogenic acid were detected in the range of 5.05 ~ 80.15μg.mL-1 (r = 0.999 1), 5.00-80μg.mL-1 (r = 0.999 7), 0.53-8.12 μg.mL-1 (r = 0.999 2), 5.30 ~ 80.12 μg.mL-1 (r = 0.999 2), and the peak area showed a good linear relationship. The recoveries (n = 9) were 100.5%, 101.6%, 103.4% and 101.8%, respectively. The RSDs were 4.8%, 7.4%, 2.7% and 6.3%, respectively. Conclusion The method is fast, simple, accurate, reproducible and suitable for the quality control of