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目的:通过口服低剂量抗原(rhβ2GP1)干预EAPS疾病进展过程中小鼠外周血CD4+CD25+Treg和Th17数量和功能变化,探讨EAPS口服耐受机制。方法:ELISA法检测小鼠外周血浆中IL-17、IL-2、IL-6、TGF-β水平变化;计算小鼠流产率(%)、活化部分凝血活酶时间(APTT)和血小板计数(PLT)判断APS病理改变;流式细胞术检测小鼠PBMC中CD4+CD25+Treg及Th17细胞变化。结果:与模型组相比,耐受组小鼠IL-17、IL-2、IL-6水平明显降低,APTT缩短,TGF-β、PLT升高,流产率降低,均差异显著(P<0.05);耐受组小鼠PBMC中CD4+CD25+Treg和Th17细胞百分率及两者的比率与模型组相比差异显著(P<0.05),而与对照组则无差异(P>0.05)。结论:CD4+CD25+Treg/Th17在EAPS口服耐受的诱导中发挥重要作用。
OBJECTIVE: To investigate the effects of oral administration of low dose rhβ2GP1 on the number and function of CD4 + CD25 + Treg and Th17 in peripheral blood of EAPS mice during the progression of EAPS. Methods: The levels of IL-17, IL-2, IL-6 and TGF-β in the peripheral blood of mice were detected by ELISA. The miscarriage rate, APTT and platelet count PLT) to determine the pathological changes of APS. The changes of CD4 + CD25 + Treg and Th17 cells in PBMC of mice were detected by flow cytometry. Results: Compared with the model group, the levels of IL-17, IL-2 and IL-6 in the tolerated group were significantly decreased, the APTT was shortened, the levels of TGF-β and PLT were increased and the abortion rate was decreased ). The percentages of CD4 + CD25 + Treg and Th17 cells in the PBMC of the tolerated group were significantly higher than those in the model group (P <0.05), but not in the control group (P> 0.05). Conclusion: CD4 + CD25 + Treg / Th17 plays an important role in the induction of oral tolerance of EAPS.