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目的观察二烯丙基三硫化物涂层支架对犬冠状动脉损伤后细胞凋亡和凋亡相关基因蛋白Bcl-x1表达的影响。方法犬进行冠状动脉左回旋支支架植入术,将二烯丙基三硫化物涂层支架和对照支架分别植入左回旋支的远端和近端,术后28d处死,进行细胞凋亡检测、Bcl-x1免疫组化和蛋白质印迹杂交。结果术后28d,二烯丙基三硫化物涂层支架组细胞凋亡率为(9.18±3.41)%高于对照支架组的(3.28±1.74)%,P<0.01。对照支架组血管壁内Bcl-x1蛋白表达为,12.17±5.02,显著高于二烯丙基三硫化物涂层支架组的5.46±2.50,P<0.05。蛋白质印迹杂交也显示对照支架组有高水平Bcl-x1蛋白表达(37.52±6.15),明显高于二烯丙基三硫化物涂层支架组(10.48±2.75),P<0.01。结论二烯丙基三硫化物可逆转血管损伤后内膜中Bcl-x1的高表达,促进细胞凋亡,可能是二烯丙基三硫化物涂层支架抑制内膜增生的机制之一。
Objective To observe the effect of diallyl trisulfide (TDS) -coated stent on the apoptosis and the expression of Bcl-x1 protein after coronary artery injury in dogs. Methods Dogs were implanted with left circumflex coronary artery. The diallyl trisulfide-coated scaffolds and control scaffolds were respectively implanted into the distal and proximal left circumflex branches and sacrificed 28 days after surgery to detect apoptosis , Bcl-xl immunohistochemistry and Western blot hybridization. Results The apoptotic rate of diallyl trisulfide-coated stent group (9.18 ± 3.41)% was significantly higher than that of the control group (28 ± 1.74)% at 28 d after operation (P <0.01). The expression of Bcl-x1 in the vascular wall of the control group was 12.17 ± 5.02, which was significantly higher than that of the diallyl trisulfide-coated stent group (5.46 ± 2.50, P <0.05). Western Blot also showed that the control group had a high level of Bcl-x1 protein expression (37.52 ± 6.15), which was significantly higher than that of the diallyl trisulfide-coated stent group (10.48 ± 2.75), P <0.01. Conclusion Diallyl trisulfide can reverse the high expression of Bcl-x1 in the intima after vascular injury and promote apoptosis. It may be one of the mechanisms of diallyl trisulfide-coated stent inhibiting intimal hyperplasia.