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目的:探讨胆管癌患者血管内皮生长因子C和D(vascular endothelial growth factor-C and-D,VEGF-C and VEGF-D)在胆管癌组织中的表达及其与肿瘤淋巴结转移的关系。方法:应用免疫组化SABC法及Real-time PCR法检测57例胆管癌组织和正常胆管组织中VEGF-C、VEGF-D蛋白及其mRNA的表达。结果:胆管癌组织VEGF-C和VEGF-D表达明显高于正常胆管组织(P<0.01),其中淋巴结转移组VEGF-C、VEGF-D的表达与淋巴结未转移组间统计学差异显著(P<0.05)。VEGF-C和VEGF-D在胆管癌组织中的表达与淋巴结转移有关(P<0.01)。结论:胆管癌细胞非摄入性高表达的VEGF-C和VEGF-D与淋巴结转移密切相关,可作为评估胆管癌患者预后的重要参考指标。
OBJECTIVE: To investigate the expression of vascular endothelial growth factor-C and VEGF-C and VEGF-C in cholangiocarcinoma and its relationship with lymph node metastasis in patients with cholangiocarcinoma. Methods: The expressions of VEGF-C, VEGF-D and their mRNA in 57 cases of cholangiocarcinoma and normal bile duct tissues were detected by immunohistochemical SABC method and Real-time PCR method. Results: The expressions of VEGF-C and VEGF-D in cholangiocarcinoma were significantly higher than those in normal cholangiocarcinoma (P <0.01). The expressions of VEGF-C and VEGF-D in lymph node metastasis were significantly different from those in lymph node metastasis <0.05). The expression of VEGF-C and VEGF-D in cholangiocarcinoma was correlated with lymph node metastasis (P <0.01). Conclusion: The high non-inducible expression of VEGF-C and VEGF-D in cholangiocarcinoma cells is closely related to the lymph node metastasis, which can be used as an important reference index to evaluate the prognosis of cholangiocarcinoma patients.