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目的：分析重组人血小板第４因子（ｒｈＰＦ４）的生物学活性。方法：采用小鼠骨髓巨核细胞以及人巨核细胞白血病细胞株，从体内和体外多方面比较天然ＰＦ４和ｒｈＰＦ４的生物学活性。结果：此ｒｈＰＦ４具有与天然ＰＦ４相同的抑制小鼠骨髓巨核细胞祖细胞生长、减少集落形成的能力，这种抑制作用能被肝素中和；ｒｈＰＦ４对人巨核细胞白血病细胞株Ｍｅｇ－０１生长也有抑制作用。结论：ｒｈＰＦ４可取代天然ＰＦ４应用于临床及科研。
Objective: To analyze the biological activity of recombinant human platelet factor 4 (rhPF4). Methods: The biological activities of native PF4 and rhPF4 were compared in vitro and in vivo using mouse bone marrow megakaryocytes and human megakaryocyte leukemia cell lines. RESULTS: This rhPF4 had the same ability to inhibit the growth of mouse bone marrow megakaryocytic progenitor cells and reduce the colony formation as native PF4. This inhibition could be neutralized by heparin. RhPF4 also inhibited the growth of human megakaryocyte leukemia cell line Meg-01 effect. Conclusion: rhPF4 can replace natural PF4 for clinical and scientific research.