近年来,我国临床胚胎姨岛移植发展迅速并取得可喜的结果。但是,实验性胰岛移植的研究工作却远为落后。主要原因是胰岛分离在方法学上存在一定困难。为了深入进行胰岛移植的研究,特别是进行移植免疫学研究及探讨排斥反应机理及抗排斥措施,要求解决纯系动物的胰岛分离及移植方法。与此同时,分离胰岛的方法也是开展胰腺内分泌学,胰岛生理学及免疫生物学研究的有力手段。对胰岛分离的方法,已有许多研究。自1967年Lacy发表胶元酶消化分离胰岛的方法以来,许多实验室都在利用这种方法。鉴于国内尚未见有关纯系小鼠胰岛移植的研究报告,我们参照Sutherland实验室的方 In recent years, the transplantation of clinical embryos in our country has developed rapidly and gratifying results have been achieved. However, the research on experimental islet transplantation is far behind. The main reason is that there is some methodological isthmus for islet isolation. In order to further study on islet transplantation, especially for transplant immunology research and to explore the mechanism of rejection and anti-rejection measures, it is required to solve the islet isolation and transplantation method of pure-line animals. At the same time, the method of isolating islets is also a powerful tool for the study of pancreatic endocrinology, islet physiology and immunobiology. There have been many studies on methods of islet isolation. Many laboratories have been using this method since Lacy published the method of collagenase digestion of islets in 1967. In view of the fact that there is no report about the islet transplantation in pure mice in China, we refer to Sutherland’s laboratory