本文报告一种新型非同位素免疫分析法,非标记稀土铕配合物荧光免疫分析.它仍以免疫反应为基础,所不同的是无须制备标记物.较标记免疫分析法简便、快速.在所建立的方法中,金属硫蛋白(MT)的量与Eu-β-二酮配合物的荧光强度成负相关.测定范围为0～1.3μg/mL,测定血清中MT的回收率为95%～100%,相对标准偏差<10%. In this paper, we report a new non-isotopic immunoassay, a fluorescence immunoassay for non-labeled rare earth europium complexes, which is based on an immunoassay, but does not require the preparation of labels, and is simple and rapid compared to the labeled immunoassay , The amount of metallothionein (MT) was negatively correlated with the fluorescence intensity of Eu-β-diketone complex in the range of 0-1.3 μg / mL, and the recovery of MT in serum was 95% -100 %, Relative standard deviation <10%.