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目的:研究17-丙烯胺-17去甲氧格尔德霉素(17-Allylamino-17-emethoxy-geldanamycin,17-AAG)对球囊损伤后大鼠颈动脉血管平滑肌细胞增殖的影响。方法:选取雄性Sprague-Dawley(SD)大鼠30只,随机数字法分为球囊损伤(Balloon injury,BI)组10只、球囊损伤+17-AAG低剂量治疗(Balloon injury+Low dose AGG,BIL)组10只、球囊损伤+17-AAG高剂量治疗(Balloon injury+High dose AGG,BIH)组10只。建立大鼠颈动脉球囊损伤模型,病理学评估血管内膜增生情况。各组于球囊损伤28天后取材,将损伤区域的血管段取材固定,苏木精-伊红染色(HE)观察血管并测量内膜面积(Intimal Area,IA)、中膜面积(Membrane Area,MA),计算内膜/中膜面积比(IA/MA),以评估内膜增生情况;同时,利用免疫荧光染色(Immunofluorescence staining,IFS)观察增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)表达的情况,以评价血管平滑肌细胞的增殖情况。结果:大鼠颈动脉球囊损伤模型成功建立。HE染色后计算血管I/M比值,结果表明BIL组与BI组血管I/M比值无统计学差异(P>0.05),BIH与BI组血管I/M比值有统计学差异(P<0.05)。IFS结果表明,BIL组血管壁PCNA表达较BI组略有降低,但无统计学意义(P>0.05)。BIH组血管壁PCNA表达较BI组明显减低,有显著统计学差异(P<0.05)。结论:一定浓度的17-AAG可明显的抑制球囊损伤后颈动脉血管平滑肌细胞增殖;17-AAG可以成为球囊损伤后大鼠血管平滑肌细胞增殖的抑制剂。
Objective: To study the effect of 17-Allylamino-17-emethoxy-geldanamycin (17-AAG) on the proliferation of rat carotid artery smooth muscle cells after balloon injury. Methods: Thirty male Sprague-Dawley (SD) rats were randomly divided into balloon injury (Bal) group (10 rats), balloon injury + 17-AAG low dose , 10 in BIL group and 10 in Balloon injury + High dose AGG (BIH) group. The carotid artery balloon injury model was established in rats, and pathological evaluation of intimal hyperplasia was performed. All the rabbits were sacrificed 28 days after balloon injury, and the vascular segments in the injured area were harvested and fixed. The blood vessels were observed by hematoxylin-eosin staining and the intimal area (IA) and mesencephalic area (MA). The intimal / media area ratio (IA / MA) was calculated to evaluate the intimal hyperplasia. Meanwhile, the expression of Proliferating cell nuclear antigen (PCNA) was detected by immunofluorescence staining (IFS) Situation to evaluate the proliferation of vascular smooth muscle cells. Results: The carotid artery balloon injury model was successfully established in rats. The ratio of vascular I / M in BIL group and BI group showed no significant difference (P> 0.05). The ratio of vascular I / M in BIH and BI group had statistical significance (P <0.05) . The results of IFS showed that the expression of PCNA in BIL group was slightly lower than that in BI group, but not statistically significant (P> 0.05). The expression of PCNA in BIH group was significantly lower than that in BI group, with significant difference (P <0.05). CONCLUSION: 17-AAG can inhibit the proliferation of smooth muscle cells of carotid artery after balloon injury. 17-AAG can be used as an inhibitor of vascular smooth muscle cell proliferation after balloon injury.