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使用无金属博莱霉素和博莱霉素的铜、钴复合物处理中华地鼠V_(49)。细胞,观察对DNA链断裂及细胞集落形成的影响。方法是选用单层中华地鼠V_(49)细胞培养一天,将细胞标本用~8H-胸腺嘧啶标记15～16小时,再用博莱霉素处理细胞标本一小时,经胰酶消化10～20分钟,冷冻离心,取沉淀加入溶解液,小心地避光溶解15分钟,超声波打碎10秒钟,再用羟磷石灰进行柱层折,
The Chinese hamster V_(49) was treated with a copper-cobalt complex without metal bleomycin and bleomycin. Cells, observe the effect on DNA strand breaks and cell colony formation. The method is to use a single layer of Chinese hamster V_ (49) cells cultured for one day, cell specimens labeled with ~8H-thymidine for 15 to 16 hours, and then treated with bleomycin for one hour, trypsin digestion 10 to 20 Minutes, refrigerated by centrifugation, took precipitate to dissolve the solution, carefully protected from light for 15 minutes, ultrasonically smashed for 10 seconds, then column layer fold with hydroxyapatite lime,