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有报道表明,母体过多的锰可通过胎盘进入胎儿体内,并引起胎儿脑内某些微量元素的变化,特别是额叶皮质的微量元素Zn含量明显减少,但神经元形态结构是否有变化,尚未见有报道.为了研究孕期锰接触对仔代中枢神经系统发育的影响,本实验选用健康成年Wistar大鼠65只,体重250g,分成4组,于妊娠第一天开始染毒,隔天经腹腔注射氯化锰水溶液,剂量分别为3.75、7.5、15mgMn/kg MnCL_2,共10次.对照组则注射等量的生理盐水.自然分娩.用亚细胞形态立体计量学方法对其仔代额叶皮质神经元线粒体的6个形态参数进行统计分析.结果发现,7.5mgMn/kg染锰组与15mg Mn/kg染锰组的仔鼠其线粒体的体密度Vv、脊膜比膜面Sm_2,内膜比膜面Sm_1、内膜面密度Sv_1,脊膜面密度SV_3,及外膜比表面S_2均有显著性减少;线粒体的面数密度NA_1则无明显改变.结果提示:孕期接触一定量的锰,将引起其
It is reported that excessive maternal manganese can enter the fetus through the placenta and cause some changes in fetal brain trace elements, especially in the frontal cortex of trace elements Zn decreased significantly, but whether there are changes in neuronal morphology, In order to study the impact of manganese exposure during pregnancy on the generation of central nervous system development, 65 healthy adult Wistar rats weighing 250g were divided into 4 groups and were exposed to the first day of gestation. Intraperitoneal injection of aqueous solution of manganese chloride, the doses were 3.75,7.5,15 mg Mn / kg MnCL 2, a total of 10 times.The control group was injected with the same amount of saline.Natural childbirth.Using sub-cellular morphology stereotaxic method on behalf of their frontal lobe Six morphological parameters of mitochondria in cortical neurons were statistically analyzed.It was found that the mitochondrial body density Vv, membrane-spanning membrane Sm_2, intima of 7.5 mgMn / kg Mn-Zn group and 15 mg Mn / kg Mn- The surface density of Sm_1, Sv_1, SV_3 and the outer surface S_2 of mitochondria were significantly decreased.The number density of mitochondria NA_1 did not change significantly.The results suggest that during pregnancy exposure to a certain amount of manganese, Will cause it