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目的 探讨儿童原发性肾病综合征 (NS)淋巴细胞免疫功能的动态变化 ,检测可溶性白介素 2受体 (sIL - 2R)和T细胞亚群 ,阐明其临床意义。方法 应用酶联免疫吸附试验 (ELISA)方法对 2 5例NS活动期和 2 0例缓解期患儿的血清和尿液检测sIL - 2R浓度。应用流式细胞仪检测T细胞亚群的变化。结果 ①NS活动期组血清和尿液sIL - 2R浓度分别高于缓解期组 (P <0 .0 1 )和对照组 (P<0 .0 1 ) ;②NS活动期组CD3+ 、CD4+ 、CD4+ /CD8+ 比值、NK[CD(1 6 +56) ] + 细胞均明显低于缓解期组 (P <0 .0 1 )和对照组 (P <0 .0 1 )。结论 NS细胞免疫功能减低 ,血和尿中sIL - 2R浓度的变化可作为肾病综合征活动指标之一。
Objective To investigate the dynamic changes of lymphocyte immune function in children with primary nephrotic syndrome (NS) and to evaluate the clinical significance of soluble interleukin 2 receptor (sIL - 2R) and T cell subsets. Methods Serum and urine samples from 25 children with active NS and 20 normal controls were tested for serum concentration of sIL - 2R by enzyme - linked immunosorbent assay (ELISA). The changes of T cell subsets were detected by flow cytometry. Results ① The levels of sIL - 2R in serum and urine in NS active stage were higher than those in remission stage (P <0.01) and control group (P <0.01). ② The levels of CD3 +, CD4 +, CD4 + / CD8 + Ratio, NK [CD (16 +56)] + cells were significantly lower than those in remission group (P <0.01) and control group (P <0.01). Conclusion The decrease of immune function of NS cells and the change of sIL - 2R in blood and urine can be used as one of the indicators of nephrotic syndrome.