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目的研究中国自然人群解偶联蛋白2-866G/A多态性与肥胖的关系。方法调查对象为山西省盂县和北京石景山2组人群共3735人,采用标准方法测量身高、体重、腰围,计算体重指数,同时调查其他心血管病危险因素。分别采用DNA MassARRAY技术和PCR-RFLP方法检测2人群UCP2-866G/A位点多态性。以资料完整且排除癌症,心肌梗塞,脑卒中和糖尿病后的2817人为研究对象。使用t检验、χ2检验、一般线性模型(generalized linear model,GLM)和Logistic回归模型分析研究对象解偶联蛋白2-866G/A各基因型及等位基因与肥胖相关指标的关系。结果研究人群UCP2-866G/A基因型频率为AA0.221、AG0.496、GG0.284,基因型分布符合Hardy-Weinberg平衡(P>0.05),石景山和盂县人群基因型频率差异无统计学意义(P=0.508),性别组间基因型频率差异有统计学意义(P=0.032)。男性肥胖组和对照组的基因型分布频率有显著性差异(χ2=8.046,P=0.018),但等位基因频率差异没有统计学意义(χ2=2.945,P=0.086)。GLM分析调整地区,年龄,体力活动、吸烟饮酒、血脂异常后,男性肥胖组中该基因多态性与腰围有关(P=0.007),携带GG基因型的男性肥胖者具有更大的腰围;男性腹型肥胖组中该基因多态性与BMI(P=0.017)和腰围(P=0.015)均有关,携带GG基因型的男性腹型肥胖者具有更大的BMI和腰围。Logistic回归分析调整地区,年龄,体力活动、吸烟饮酒、血脂异常后,男性-866G/A多态性与肥胖发生有关(P=0.018),男性中GG基因型个体相对AA基因型具有更高的肥胖率,但是没有统计学意义(OR=1.36,95%CI:0.82-2.258,P=0.234)。结论中国男性人群解偶联蛋白2启动子区866G/A多态性可能与肥胖有关。
Objective To study the relationship between polymorphism of uncoupling protein 2-866G / A and obesity in Chinese natural population. Methods The subjects were 3735 people from Yuxian County, Shanxi Province and Shijingshan City, Beijing. The height, weight and waist circumference were measured by standard methods and the body mass index was calculated. Other cardiovascular risk factors were investigated. The polymorphism of UCP2-866G / A in two populations was detected by DNA MassARRAY and PCR-RFLP respectively. A total of 2,817 people were enrolled in this study after complete data and excluding cancer, myocardial infarction, stroke and diabetes. The t-test, χ2 test, generalized linear model (GLM) and logistic regression model were used to analyze the relationship between genotypes and alleles of 2-866G / A and obesity-related indicators. Results The frequency of UCP2-866G / A genotype was AA0.221, AG0.496, GG0.284, and the genotype distribution was in accordance with Hardy-Weinberg equilibrium (P> 0.05). There was no significant difference in genotype frequency between Shijingshan and Yuxian Significance (P = 0.508), genotype frequencies between genders were significantly different (P = 0.032). There was a significant difference in genotype frequencies between male obesity group and control group (χ2 = 8.046, P = 0.018), but there was no significant difference in allele frequency (χ2 = 2.945, P = 0.086). GLM analysis of the adjustment for regional, age, physical activity, smoking, drinking, dyslipidemia, male obesity group, the gene polymorphism and waist circumference (P = 0.007), GG genotype male obese with larger waist circumference; men The polymorphism of this gene was associated with BMI (P = 0.017) and waist circumference (P = 0.015) in abdominal obesity group, and the male with abdominal GG genotype had greater BMI and waist circumference. Logistic regression analysis showed that male -866G / A polymorphism was associated with obesity (P = 0.018) after adjusting for regional, age, physical activity, smoking, drinking, and dyslipidemia. Individuals with GG genotype had higher Obesity rates, but not statistically significant (OR = 1.36, 95% CI: 0.82-2.258, P = 0.234). Conclusion The 866G / A polymorphism of the uncoupling protein 2 promoter in Chinese male population may be related to obesity.