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为了解子宫内膜表面复合糖在胚泡着床过程中的作用，作者应用具不同寡糖特异性的单克隆抗体为工具，观察了小鼠子宫内膜岩藻糖化抗原的结构特点、分布和在不同时期的变化。荧光免疫组化分析的结果表明：（１）子宫内膜上皮细胞及腺细胞表面有ＬｅＸ、ＬｅＹ及Ｈ－１三种岩藻糖化抗原存在；（２）在动情期ＬｅＹ及Ｈ－１抗原可进入子宫腔中；（３）着床期ＬｅＹ抗原的表达有增强趋势，但Ｈ－１抗原则减弱，且在细胞表面呈不连续的簇状分布。通过ＳＤＳ一ＰＡＧＥ及印迹电泳还观察到：（１）未孕及早孕的子宫内膜均有多种含ＬｅＹ糖链的蛋白质（ＬｅＹ一糖蛋白）；（２）早孕第４天的内膜有阶段特异性ＬｅＹ糖蛋白（分子量１２～１６Ｘ１０３）出现，这与子宫接受胚泡植入的时期相应。结果提示上述阶段特异性糖抗原和胚泡与子宫内膜的粘连、植入有关。
In order to understand the role of endometrial polysaccharide in blastocyst implantation, the authors used monoclonal antibodies with different oligosaccharides specificity as a tool to observe the structural characteristics, distribution and expression of fucosylated antigen in mouse endometrium Changes in different periods. The results of fluorescence immunohistochemical analysis showed that: (1) there are three fucosylated antigens of LeX, LeY and H-1 on the surface of endometrial epithelial cells and glandular cells; (2) LeY and H-1 antigens can enter during estrus Uterine cavity; (3) implantation of LeY antigen expression trend, but the H-1 antigen is weakened, and in the cell surface was discontinuous cluster distribution. SDS-PAGE and western blotting also showed that: (1) There are many LeY-glycoproteins in the endometrium of non-pregnant and early pregnant women; (2) Intima of the fourth day of early pregnancy Phase-specific LeY glycoprotein (molecular weight 12 ~ 16X103) appeared, which corresponds to the period of uterine implantation of blastocysts. The results suggest that the stage of specific carbohydrate antigen and blastocyst adhesion and implantation of the endometrium.