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我们建立了完全去除植物性神经的肝郁气滞动物模型,并应用抗囊泡乙酰胆碱载体蛋白(VAChT)单克隆抗体和免疫组化技术建立了神经去除程度的评价方法,从形态学上观测肝脏神经的去除程度,并在实验期间观察神经有否再生现象。在此基础上,测定大鼠空腹及进食后去神经支配肝脏的糖原含量及肝纤维化4项指标,以探讨植物性神经对肝脏葡萄糖代谢和
We established a model of liver-qi stagnation completely removing vegetative nerves and established a method for evaluating the degree of neural removal using monoclonal antibody against VAChT and immunohistochemistry to observe the morphological changes of the liver The degree of nerve removal, and observed during the experiment whether nerve regeneration phenomenon. On this basis, the determination of fasting and eating rats after denervation of liver glycogen content and liver fibrosis 4 indicators to explore the autonomic nerve on liver glucose metabolism and