论文部分内容阅读
目的探讨丹参联合放疗对小鼠Lewis肺癌肿瘤组织中微血管密度(MVD)的影响。方法建立Lewis肺癌荷瘤鼠模型后,随机分为模型组、丹参低剂量组(丹低组)、丹参高剂量组(丹高组)、放疗组、丹低+放疗组(联合1组)、丹高+放疗组(联合2组),每组各10只小鼠。各组于接种第二日起每天腹腔注射给药,连续12 d,放疗组、联合1组、联合2组于接种第7日起给予钴-60照射肿瘤局部,放疗剂量2 Gy.d-1,连续5 d。末次给药24 h后处死小鼠,剥离肿瘤组织称重,计算抑瘤率;免疫组化法检测肿瘤组织中微血管密度(MVD)。结果丹参低剂量联合放疗组的微血管密度较放疗组和丹参低剂量组明显降低(P<0.05)。结论丹参联合放疗对Lewis肺癌小鼠肿瘤血管生成有明显的抑制作用。
Objective To investigate the effect of radix salviae miltiorrhizae combined with radiotherapy on microvessel density (MVD) in Lewis lung carcinoma of mice. Methods Lewis lung cancer tumor-bearing mice model was established and randomly divided into three groups: model group, low dose Salvia miltiorrhiza group (Dan low group), high dose Salvia miltiorrhiza group (Dan high group), radiotherapy group, Dan low + radiotherapy group Dan high + radiotherapy group (combination of 2 groups), each group of 10 mice. The rats in each group were administered intraperitoneally on the second day after inoculation for 12 days. The radiotherapy group, the combination group and the combination group were given cobalt-60 irradiation on the 7th day after inoculation. The radiation dose was 2 Gy.d-1 For 5 days. Twenty-four hours after the last administration, the mice were sacrificed and the tumor tissue was peeled off to calculate the tumor inhibition rate. The MVD was measured by immunohistochemistry. Results The microvessel density of Salvia miltiorrhiza combined with low-dose radiotherapy group was significantly lower than that of radiotherapy group and Salvia miltiorrhiza low-dose group (P <0.05). Conclusion Radix Salviae Miltiorrhizae combined with radiotherapy significantly inhibited the tumor angiogenesis in mice with Lewis lung cancer.