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为探究小麦偏分离规律及给小麦农艺性状的QTL定位研究提供相关信息,对普通小麦Heyne×Lakin的145个重组自交系的2 241个标记基因型(包括2 071个SNP标记和170个SSR标记)进行连锁作图及偏分离分析,检测SNP和SSR标记偏分离的热点区域(SDR)。结果表明,共有2 197个标记(占总标记数的98.0%)定位在覆盖小麦21条染色体的42个连锁群中,连锁遗传图谱的总长度为2 265.9cM,标记间的平均遗传距离为1.03cM。在连锁遗传图谱中,共有230个标记位点表现偏分离,占总标记数的10.5%。其中164个标记偏向父本Lakin,占偏分离标记数的71.3%;66个标记偏向母本Heyne,占偏分离标记数的28.7%。检测到11个SDRs,分别位于2A、2B、2D、3B、5D、6B和7A染色体上,其中有5个SDRs偏向母本Heyne,6个SDRs偏向父本Lakin,推测这些偏分离热点区域的形成可能与配子体选择有关。
In order to explore the rule of partial segregation of wheat (Triticum aestivum L.) and to provide QTL mapping of agronomic traits in wheat, 2 241 marker genotypes (including 2 071 SNPs and 170 SSRs) of 145 recombinant inbred lines of common wheat Heyne × Lakin Markers) for linkage mapping and partial segregation analysis to detect hot spots (SDRs) with partial segregation of SNPs and SSR markers. The results showed that a total of 2 197 markers (98.0% of the total number of markers) were located in 42 linkage groups covering 21 wheat chromosomes. The total length of the linkage map was 2 265.9 cM, and the average genetic distance between markers was 1.03 cM. In the linkage map, a total of 230 markers showed partial segregation, accounting for 10.5% of the total number of markers. Lakin was the dominant marker in 164 markers, accounting for 71.3% of the total number of segregation markers; and 66 markers favor Heyne, accounting for 28.7% of the number of segregation markers. Eleven SDRs were detected on chromosomes 2A, 2B, 2D, 3B, 5D, 6B and 7A respectively. Among them, 5 SDRs were biased toward Heyne and 6 SDRs were biased toward male parent Lakin. May be related to gametophyte selection.