本文阐述了质谱法在蛋白质及多肽的氨基酸序列分析中的适用范围及与酶学法、化学法、X-晶体衍射法等传统方法相比较的优点。着重介绍质谱法与各种分析方法综合运用于微量蛋白质结构的测定和质谱技术在该领域中的应用前景。 This article describes the application of mass spectrometry in the amino acid sequence analysis of proteins and peptides and compares with the traditional methods such as enzymatic method, chemical method and X-ray diffraction method. It focuses on the application of mass spectrometry and various analytical methods combined with the determination of trace protein structure and the application prospect of mass spectrometry in this field.