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目的探讨中国陕西地区汉族人群花生四烯酸15-脂加氧酶(arachidonate 15-lipoxygenase,ALOX15)基因多态性是否是冠心病(coronary heart disease,CHD)的遗传易感因素,从而为该病的早期诊断和预防提供相关依据。方法应用聚基质辅助激光解吸附电离飞行时间质谱方法(matrix-assisted laser desorption/ionization-time of flight,MALDI-TOF),检测105例CHD患者(CHD组)和75例年龄及性别相匹配的非冠心病者(对照组)的ALOX15基因3个位点rs916055、rs2619112、rs2664593的单核苷酸多态性(single nucleotide polymorphisms,SNPs),判定其基因型并统计各基因型及等位基因的频率。结果 rs916055A/G的基因型以及等位基因频率在CHD组和对照组间有统计学差异(P=0.000 1,P=0.000 1);rs2619112A/G的基因型以及等位基因频率在CHD组和对照组间无统计学差异(P=0.134 2,P=0.143 8);rs2664593C/G的基因型在CHD组和对照组间有统计学差异(P=0.002 7),等位基因频率在CHD组和对照组间无统计学差异(P=0.537 1);应用Logistic回归分析调整了其他相关因素后显示,rs916055位点携带A等位基因为CHD发病的独立危险因素。结论 rs916055位点可能与CHD发病相关,A等位基因可能是CHD的遗传易感基因。
Objective To investigate whether genetic polymorphism of arachidonate 15-lipoxygenase (ALOX15) is a genetic predisposition to coronary heart disease (CHD) in Han population in Shaanxi Province, China. Provide early diagnosis and prevention related basis. Methods 105 cases of CHD (CHD group) and 75 age-matched and non-sex matched non-human matched subjects (CHD group) were detected by matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) The SNPs of rs916055, rs2619112 and rs2664593 at the three loci of ALOX15 gene in patients with coronary heart disease (control group) were genotyped and the frequencies of genotypes and alleles were calculated . Results The genotype and allele frequency of rs916055A / G were significantly different between CHD group and control group (P = 0.000 1, P = 0.000 1). The rs2619112A / G genotype and allele frequency were significantly different between CHD group and control group The genotype of rs2664593C / G was significantly different between the CHD group and the control group (P = 0.002 7), and the allele frequency in the CHD group was significantly higher than that in the control group (P = 0.134 2, P = 0.143 8) (P = 0.537 1). Logistic regression analysis adjusted other related factors and showed that the rs916055 A allele was an independent risk factor for CHD. Conclusion The rs916055 locus may be related to the pathogenesis of CHD. The allele A may be a genetic predisposition to CHD.