论文部分内容阅读
目的探讨非小细胞肺癌(NSCLC)行肺癌根治术患者的外周血LUNX m RNA表达,及其对判断预后的价值。方法选择2013年3月至2014年3月于我科住院治疗的NSCLC患者共200例,用Trizol法提取其外周血总RNA,并进行逆转录及PCR扩增和荧光测定PCR反应,术后随访其复发及转移的情况。结果 142例外周血LUNX表达阳性者复发98例,复发率为69.0%;58例外周血LUNX表达阴性者复发8例,复发率为13.8%,明显低于外周血LUNX表达阳性者复发的比例,差异有统计学意义,P<0.05。结论 NSCLC患者外周血中LUNX阳性者,其复发率高、预后较差。
Objective To investigate the expression of LUNX mRNA in peripheral blood of non-small cell lung cancer (NSCLC) patients undergoing radical resection of lung cancer and its value of prognosis. Methods A total of 200 patients with NSCLC who were hospitalized in our department from March 2013 to March 2014 were selected. Total RNA was extracted from the peripheral blood by Trizol method and subjected to RT-PCR, PCR amplification and PCR reaction. The patients were followed up Its recurrence and metastasis. Results The recurrence rate was 69.0% in 142 cases of peripheral blood LUNX-positive patients. The recurrence rate was 13.8% in 58 cases of peripheral blood LUNX-negative patients, which was significantly lower than that of patients with LUNX-positive recurrence, The difference was statistically significant, P <0.05. Conclusion In patients with NSCLC, the positive rate of LUNX in the peripheral blood is high, and the prognosis is poor.