论文部分内容阅读
采用RT-PCR技术对采自中国湖北、浙江和山东的91份芋[Colocasia esculenta(L.)Schott]样品的芋花叶病毒(Dasheen mosaicvirus,DsMV)进行了检测,检出率为26.4%。对其中14个DsMV分离物的317bp扩增产物(为外壳蛋白基因的一部分)序列分析的结果显示,各分离物内的核苷酸变异相对较低,而分离物间存在较大的分子变异,相似性为68.3%~97.8%。对来自湖北和浙江的2个DsMV分离物DsMV-SCS和DsMV-JH的外壳蛋白基因(coatproteingene,cp)进行了测序,全长分别为951bp和987bp,二者cp核苷酸和氨基酸序列相似性分别为79.0%和82.3%,与已报道DsMV的cp核苷酸和氨基酸序列相似性分别为73.0%~92.1%和74.8%~98.2%,在构建的系统发育树上聚在两个不同的簇,各DsMV分离物的系统进化关系与其寄主和地理来源无显著相关性。
RT-PCR was used to detect Dasheen mosaic virus (DsMV) in 91 samples of Colocasia esculenta (L.) Schott collected from Hubei, Zhejiang and Shandong of China. The detection rate was 26.4%. Sequence analysis of the 317 bp amplification product (which is part of the coat protein gene) of 14 DsMV isolates showed that the nucleotide variation within each isolate was relatively low, whereas there was a large molecular variance among the isolates. The similarity is 68.3% ~ 97.8%. The coat protein genes (cp) of DsMV-SCS and DsMV-JH from two DsMV isolates from Hubei and Zhejiang were sequenced and their full-length were 951bp and 987bp, respectively. The nucleotide and amino acid sequence identities 79.0% and 82.3% respectively. The similarity of nucleotide and amino acid sequence of cp to DsMV was 73.0% -92.1% and 74.8% -98.2%, respectively. The two phylogenetic trees clustered in two different clusters The phylogenetic relationships among DsMV isolates were not significantly related to their host and geographical origin.