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目的：检测一种恶性疟原虫保护性抗原重组复合基因表达产物（ＨＲＡ）诱导小鼠细胞免疫的反应．方法：免疫小鼠的脾淋巴细胞，体外分别经ＣｏｎＡ、ＨＲＡ和可溶性裂殖子抗原（ＳＭＡ）的刺激，３Ｈ－ＴｄＲ参入法检测淋巴细胞的增殖水平．结果：含佐剂的ＨＲＡ免疫ＢＡＬＢ／ｃ和Ｃ５７ＢＬ／６小鼠后，其脾细胞经ＨＲＡ刺激后，增殖明显，可溶性裂殖子抗原免疫的ＢＡＬＢ／ｃ小鼠脾细胞经ＨＲＡ刺激发生明显的增殖．结论：ＨＲＡ上的Ｔ细胞位点发挥了效应．具有一定的突破ＭＨＣ限制的能力．
OBJECTIVE: To examine the response of a recombinant Plasmodium falciparum protective antigen (HRA) -induced cellular immunity in mice. Methods: The splenic lymphocytes of mice were immunized with ConA, HRA and soluble merozoite antigen (SMA) in vitro. 3H-TdR incorporation assay was used to detect the proliferation of lymphocytes. Results: BALB / c and C57BL / 6 mice were immunized with adjuvant-containing HRA, and their spleen cells proliferated significantly after being stimulated by HRA. Splenocytes of BALB / c mice immunized with soluble merozoite antigen were significantly stimulated by HRA proliferation. Conclusion: T cell sites on HRA exert effects. Has a certain ability to break the MHC limit.