我国是世界上感染乙型病毒性肝炎(以下简称乙肝)人数最多的国家,但专门调查入出境船员HBsAg携带情况的资料较少,泉州口岸的检测结果为乙肝感染的控制和预防提供了科学依据。结果报告如下: 1 材料与方法 1.1 对象 1991～1997年,在泉州卫生检疫局接受体检的13242名入出境船员。 1.2 方法 常规抽取静脉血2～3ml,采用反向被动血凝法(RPHA)进行HBsAg检测,滴度1:16以上为阳性。对HBsAg阳性者采用(ELISA)检测抗—HBs、HBeAg、抗—HBe、抗—HBc。所有试剂由上海科华实业生物技术有限公司提供,在有效期内使用。 2 结果 China is the country with the largest number of people infected with hepatitis B virus (hereinafter referred to as hepatitis B) in the world, but there is only a small amount of data on the investigation of carrying HBsAg by seafarers entering and leaving the country. The test results at Quanzhou port provide a scientific basis for the control and prevention of hepatitis B infection . The results are reported as follows: 1 Materials and Methods 1.1 Objects 1991 to 1997, Quanzhou Health and Quarantine Bureau to accept the physical examination of 13,242 crew members. 1.2 routine venous blood 2 ~ 3ml, using reverse passive hemagglutination (RPHA) for HBsAg detection, titer 1:16 or more positive. Anti-HBs, HBeAg, anti-HBe, anti-HBc were detected by ELISA on HBsAg-positive subjects. All reagents provided by Shanghai Kehua Industrial Biotechnology Co., Ltd., used within the validity period. 2 results