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目的:探讨ZD6474对肝癌细胞株HepG2和乙肝相关肝癌细胞株HepG2.2.15细胞增殖的影响。方法:用WST方法检测ZD6474对细胞增殖的抑制作用;采用流式细胞仪检测ZD6474对细胞周期及凋亡的影响。结果:ZD6474可以显著抑制HepG2和HepG2.2.15细胞增殖,6.4μmol/L的ZD6474可以抑制(46.86±10.32)%的HepG2和(41.24±7.35)%的HepG2.2.15细胞增殖,其抑制增殖作用随剂量增加而增强(P<0.05)。ZD6474诱导细胞产生G0/G1期阻滞,6μmol/L的ZD6474可诱导71.90%的HepG2和69.90%的HepG2.2.15细胞阻滞于G0/G1期。结论:ZD6474可以抑制HepG2和HepG2.2.15细胞增殖,其机制可能与诱导细胞周期阻滞有关。
Objective: To investigate the effect of ZD6474 on the proliferation of hepatocellular carcinoma cell line HepG2 and hepatitis B related hepatocellular carcinoma cell line HepG2.2.15. Methods: The inhibitory effect of ZD6474 on cell proliferation was detected by WST method. The effect of ZD6474 on cell cycle and apoptosis was detected by flow cytometry. RESULTS: ZD6474 could significantly inhibit the proliferation of HepG2 and HepG2.2.15 cells, and 6.4 μmol/L of ZD6474 could inhibit the proliferation of (46.86±10.32)% of HepG2 and (41.24±7.35)% of HepG2.2.15 cells, which inhibited proliferation with dose Increased and increased (P<0.05). ZD6474 induces G0/G1 phase arrest, and 6 μmol/L of ZD6474 can induce 71.90% of HepG2 and 69.90% of HepG2.2.15 cells to arrest in G0/G1 phase. Conclusion: ZD6474 can inhibit the proliferation of HepG2 and HepG2.2.15 cells, and its mechanism may be related to the induction of cell cycle arrest.