论文部分内容阅读
目的:观察血管抑素基因联合氟尿嘧啶腹腔化疗对预防大肠癌术后肝转移的作用.方法:RT—PCR克隆血管抑素基因,建立重组腺病毒载体.建立人结肠癌LoVo肝转移种植的模型,给予血管抑素基因联合氟尿嘧啶腹腔化疗.观察其对大肠癌细胞肝转移的影响结果:血管抑素基因联合氟尿嘧啶腹腔化疗治疗组肝转移发生率明显低于其他各组(P<0.05),肝转移瘤数少于其他各组(P<0.05),荷瘤生存时间长,治疗组肿瘤组织中血管抑素基因表达为阳性,MVD明显低于其他各组.结论:血管抑素的基因治疗与传统的腹腔化疗联合应用,对预防裸鼠人LoVo结肠癌细胞肝转移有一定的作用.
OBJECTIVE: To observe the effect of angiostatin gene combined with 5-fluorouracil intraperitoneal chemotherapy on the prevention of postoperative liver metastases in colorectal cancer.Methods: The angiostatin gene was cloned by RT-PCR and the recombinant adenovirus vector was constructed.A model of colon cancer LoVo liver metastasis was established, The effect of angiostatin gene combined with 5-fluorouracil intraperitoneal chemotherapy on hepatic metastasis of colorectal cancer cells was observed.Results: The incidence of hepatic metastasis was significantly lower in the combination of angiostatin gene and fluorouracil intraperitoneal chemotherapy than in other groups (P <0.05) The number of tumor was less than other groups (P <0.05), tumor-bearing survival time was longer, the expression of angiostatin gene was positive in tumor tissue of treatment group, MVD was significantly lower than other groups.Conclusion: The gene therapy of angiostatin and traditional Intraperitoneal chemotherapy combined with the prevention of human colon cancer LoVo cells in human liver metastasis have a certain role.