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Neural stem/progenitor cell(NSC) transplantation has been shown to effectively improve neurological function in rats with hypoxic-ischemic brain damage.Vascular endothelial growth factor(VEGF) is a signaling protein that stimulates angiogenesis and improves neural regeneration.We hypothesized that transplantation of VEGF-transfected NSCs would alleviate hypoxic-ischemic brain damage in neonatal rats.We produced and transfected a recombinant lentiviral vector containing the VEGF165 gene into cultured NSCs.The transfected NSCs were transplanted into the left sensorimotor cortex of rats 3 days after hypoxic-ischemic brain damage.Compared with the NSCs group,VEGF m RNA and protein expression levels were increased in the transgene NSCs group,and learning and memory abilities were significantly improved at 30 days.Furthermore,histopathological changes were alleviated in these animals.Our findings indicate that transplantation of VEGF-transfected NSCs may facilitate the recovery of neurological function,and that its therapeutic effectiveness is better than that of unmodified NSCs.
Neural stem / progenitor cell (NSC) has been shown to effectively improve neurological function in rats with hypoxic-ischemic brain damage. Vascular endothelial growth factor (VEGF) is a signaling protein that stimulates angiogenesis and improves neural regeneration. VEGF-transfected NSCs would alleviate hypoxic-ischemic brain damage in neonatal rats. We produced and transfected a recombinant lentiviral vector containing the VEGF165 gene into cultured NSCs. The transfected NSCs were transplanted into the left sensorimotor cortex of rats for 3 days after hypoxic-ischemic brain .Compared with the NSCs group, VEGF m RNA and protein expression levels were increased in the transgene NSCs group, and learning and memory abilities were significantly improved at 30 days. F urthermore, histopathological changes were alleviated in these animals. of VEGF-transfected NSCs may facilitate the recovery of neurologic al function, and that its therapeutic effectiveness is better than that of unmodified NSCs.