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目的 探讨慢性应激对大鼠海马CA3区锥体细胞形态结构的效应。方法 将 2 6只雄性Sprague Dawley大鼠随机分为对照组和应激组 ,每组 13只。采用尼氏 (Nissl)染色法、高尔基 (Golgi)镀染法和透射电镜技术 ,观察慢性强迫游泳应激对大鼠海马CA3区锥体细胞形态结构的效应。结果应激组大鼠海马CA3区锥体细胞数 (35 1± 3 9)较对照组 (38 7± 3 5 )明显减少 (P <0 0 5 ) ;顶树突的总长度为 (15 5 7± 33 3) μm ,较对照组 (195 6± 34 6 ) μm明显缩短 (P <0 0 5 )。应激组大鼠海马CA3区锥体细胞出现超微结构的改变 ,包括细胞固缩、体积缩小、核膜皱缩、线粒体变性和粗面内质网模糊不清。结论 慢性应激可引起海马CA3区锥体细胞形态和微细结构的改变及细胞丧失。
Objective To investigate the effect of chronic stress on the morphology of pyramidal cells in hippocampal CA3 region of rats. Methods Twenty-six male Sprague Dawley rats were randomly divided into control group and stress group, with 13 rats in each group. Nissl staining, Golgi staining and transmission electron microscopy were used to observe the effect of chronic forced swimming stress on the pyramidal cell morphology of hippocampal CA3 region in rats. Results The number of pyramidal neurons in hippocampal CA3 region of rats in stress group was significantly lower than that in control group (38 7 ± 35) (P <0.05). The total length of apical dendrites was (15 5 7 ± 33 3 μm, which was significantly shorter than that of the control group (195 6 ± 34 6 μm) (P 0 05). The ultrastructural changes of hippocampal CA3 pyramidal neurons were found in the stress group, including cell shrinkage, shrinkage, nuclear membrane shrinkage, mitochondrial degeneration and rough endoplasmic reticulum. Conclusion Chronic stress can cause changes of pyramidal cell morphology and fine structure and loss of cells in hippocampal CA3 region.