目的　利用甲醇酵母高效表达重组人血小板第 4因子 (rhPF4)。方法　用PCR方法扩增出血小板第 4因子 (PF4)的cDNA序列 ,插入到穿梭质粒pPIC9的MFα信号肽后 ,在醇氧化脱氢酶 1(AOX1)启动子的下游 ,得到重组质粒pPIC9 PF4。转化到甲醇酵母宿主菌GS115 ,经筛选得到PF4高表达菌株进行表达 ,并测定表达产物氨基酸序列和生物学功能。结果　rhPF4氨基酸序列与天然PF4相同 ,可中和肝素抗凝作用 ,并呈剂量相关。结论　rhPF4可在甲醇酵母中获得生产规模表达 ,产物性质和天然的PF4相同 Objective To express recombinant human platelet factor 4 (rhPF4) efficiently by methanol yeast. Methods The cDNA sequence of platelet factor 4 (PF4) was amplified by PCR and inserted into the MFα signal peptide of shuttle plasmid pPIC9. The downstream of the promoter of alcohol oxidase - dehydrogenase 1 (AOX1), the recombinant plasmid pPIC9 PF4 was obtained. Transformed into methanol yeast host strain GS115, screened to obtain PF4 high expression strain, and the expression product amino acid sequence and biological function were determined. Results The amino acid sequence of rhPF4 was the same as that of natural PF4, which could neutralize heparin anticoagulation and showed dose-dependent. Conclusion rhPF4 can be expressed on a production scale in methanolic yeasts with the same properties as native PF4