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本文研究了ＩＬ－１０对佐剂性关节炎（ＡＡ）大鼠腹腔巨噬细胞（Ｍφ）及脾淋巴细胞在体外诱生ＩＬ－８的影响，还探讨了ＩＬ－１０经腹腔注射后对ＡＡ大鼠的治疗作用以及在体内对ＩＬ－８合成的影响，并利用核酸杂交技术初步探讨了ＩＬ－１０的作用机制。结果表明，ＡＡ大鼠腹腔Ｍφ及脾淋巴细胞分泌ＩＬ－８的能力明显高于正常大鼠。在体外ＩＬ－１０能显著下调ＩＬ－８的表达。ＡＡ大鼠经ＩＬ－１０治疗后症状明显改善，ＩＬ－８的合成能力降低。ＩＬ－１０能明显下调正常大鼠腹腔ＭφＩＬ－８ｍＲＮＡ表达。总之，ＩＬ－１０在体内外均显著抑制ＩＬ－８的合成，并具有良好的抗炎活性，从而为临床治疗类风湿关节炎（ＲＡ）提供了新制剂。
In this paper, the effect of IL-10 on peritoneal macrophages (Mφ) and splenic lymphocytes induced by adjuvant arthritis (AA) in rats induced by IL-8 in vitro was also studied. Therapeutic effect of rat and its effect on IL-8 synthesis in vivo were studied. The mechanism of action of IL-10 was preliminarily explored by nucleic acid hybridization. The results showed that the ability of Mφ in peritoneal cavity and secretion of IL-8 by splenic lymphocytes in AA rats were significantly higher than those in normal rats. IL-10 significantly down-regulates IL-8 expression in vitro. After treatment with IL-10, the symptoms of AA rats were significantly improved, and the synthesis ability of IL-8 was decreased. IL-10 significantly down-regulated the mRNA expression of MφIL-8 in the peritoneal cavity of normal rats. In summary, IL-10 significantly inhibits the synthesis of IL-8 both in vitro and in vivo and has good anti-inflammatory activity, thus providing a novel preparation for clinical treatment of rheumatoid arthritis (RA).