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目的研究IL-16基因单核苷酸多态性(SNP)位点rs150605585 I/D在广西人群中的分布,并对比不同种族间IL-16插入/缺失频率分布的差别。方法采用单碱基延伸的聚合酶链反应(PCR)技术和DNA测序法检测303例广西人IL-16基因多态性,分析广西人群这个位点的基因型和等位基因的分布频率,并与人类基因组计划(HapMap)公布的欧洲人、中国北京汉族人、日本人和非洲人的基因多态性分型数据比较,分析这5类人群rs150605585插入/缺失的分布频率。结果在我国广西人群中存在IL-16基因rs150605585 I/D多态性,分别有I/I、I/D、D/D 3种基因型。与人类基因组计划(HapMap)公布的4类人群的SNP分型数据进行比较,IL-16基因rs150605585I/D与北京、日本人群的分布差异不具有统计学意义(P均>0.05);rs150605585I/D与非洲人、欧洲人相比差异具有统计学意义(P<0.05)。结论在广西地区人群中存在着IL-16基因多态性。广西人群IL-16基因多态性的分布与其他种族人群比较存在有差异,这种差异也许是与IL-16相关的疾病在不同地区人群间发病率不同的原因之一,这为我们研究基因与疾病的关系提供遗传数据支持。
Objective To investigate the distribution of SNP rs150605585 I / D in Guangxi population and to compare the frequency of IL-16 insertion / deletion among different races. Methods Single nucleotide polymorphism (PCR) and DNA sequencing were used to detect the polymorphism of IL-16 gene in 303 Guangxi. The genotypes and allele frequencies of this locus in Guangxi were analyzed. The frequencies of rs150605585 insertions / deletions in these 5 groups were compared with those of Han, Japanese and Africans in Beijing, China published by the Human Genome Project (HapMap). Results There was rs150605585 I / D polymorphism of IL-16 gene in Guangxi population of China, including I / I, I / D and D / D genotype. There were no significant differences in the distribution of IL-16 gene rs150605585I / D and Beijing and Japan (P> 0.05) when compared with the SNP typing data of the four groups released by the HapMap (rs150605585I / D Compared with Africans and Europeans, the difference was statistically significant (P <0.05). Conclusion There is IL-16 gene polymorphism in Guangxi population. There is a difference in the distribution of IL-16 gene polymorphism in Guangxi population compared with other ethnic groups. This difference may be one of the reasons for the different incidence of IL-16-related diseases in different regions of the population, The relationship with the disease provides genetic data support.