聚合酶链反应(PCR)是汉坦病毒特异、敏感、快速的基因检测方法,已逐步应用于临床,对流行性出血热的早期诊断意义较大.但在应用过程中,也逐步暴露出许多不完善之处,诸如容易污染、操作流程繁琐等.国内外许多学者都在积极从各个环节进行方法的优化和改进.我们尝试着将逆转录与半套式PCR第一次扩增在单管中进行,并用于临床标本检测,现报告如下: Polymerase chain reaction (PCR) is a specific, sensitive and rapid method for gene detection of Hantavirus, and has been gradually applied in clinical practice, which is of great significance in the early diagnosis of epidemic hemorrhagic fever. However, during the application, polymerase chain reaction (PCR) Imperfections, such as easy to pollute, cumbersome operation process, etc. Many scholars at home and abroad are actively carrying out the optimization and improvement of methods from all aspects.We try to first reverse transcription and semi-nested PCR amplification in a single tube In, and for clinical specimens testing, are as follows: