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用N-乙酰半胱氨酸金表面自组装技术，以及用1-乙基3-（3-二甲氨基）碳二亚胺盐酸盐（EDC）、N-羟基硫代琥珀酰亚胺（NHS）偶联剂把亲合素固定于金表面，再将生物素标记深外固定在亲合素上，来制备核酸传感器探头。然后将探头与目标DNA和第二生物素标记探针进行夹心式杂交，再利用生物素-亲合素的作用联人亲合素标记的碱性磷酸酯酶，用酶催化底物AMPPD发光来达到测定DNA的目的。测定了乙型肝炎病毒（HBV）DNA（566bp）的片段，实验测得HBV-DNA灵敏度为3×10~(-14)mol/L。
Using N-acetylcysteine gold surface-self-assembly technology, and using 1-ethyl 3- (3-dimethylamino) carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide NHS) coupling agent to immobilize the avidin on the gold surface, and then biotin-labeled immobilized on the avidin to prepare a nucleic acid sensor probe. Then the probe and the target DNA and the second biotin-labeled probe sandwich hybridization, and then the role of biotin-avidin linked to avidin-labeled alkaline phosphatase enzyme AMPPD enzyme-catalyzed luminescence to Achieve the purpose of measuring DNA. The fragment of HBV DNA (566bp) was determined. The sensitivity of HBV-DNA was 3 × 10 ~ (-14) mol / L.