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[目的]观察肺癌患者血浆及外周血单个核细胞(PBMC)诱生IL 2、IL 4水平 ,进而反映患者Th1和Th2细胞功能。[方法]运用细胞因子诱生及ELISA技术 ,测定48例肺癌术前患者和24例术后患者血清及外周血单个核细胞诱生的IL 2、IL 4水平。以IL 2水平反映Th1细胞功能 ,以IL 4水平代表Th2细胞功能。[结果]肺癌患者血清及PBMC诱生产生IL 2水平减低 ,而IL 4水平升高。PBMC诱生及血清IL 2、IL 4水平在不同分期、不同病理类型肺癌病人间比较无差别 ,行肺癌根治术后的患者血清及PBMC诱生IL 2水平高于未手术肺癌患者水平。[结论]肺癌患者体内Th1/Th2细胞亚群功能失调 ,这可能是肺癌组织在患者体内进行性生长及免疫逃避的机制之一 ;肺癌患者Th1/Th2亚群失调在肺癌不同分期、不同病理类型患者间无差别 ,而手术可改善肺癌病人Th1/Th2亚群功能失调状态。
[Objective] To observe the levels of IL 2 and IL 4 induced by plasma and peripheral blood mononuclear cells (PBMC) in patients with lung cancer, and to reflect the function of Th1 and Th2 cells. [Methods] IL-2 and IL-4 levels induced by serum and peripheral blood mononuclear cells in 48 patients with lung cancer and 24 patients after operation were determined by cytokine induction and ELISA. The level of IL 2 reflects the function of Th1 cells, and the level of IL 4 represents the function of Th2 cells. [Results] Serum and PBMC induced by lung cancer patients induced IL 2 levels to decrease, while IL 4 levels increased. There was no difference in PBMC induction and serum IL 2 and IL 4 levels between lung cancer patients with different stages and pathological types. Serum and PBMC-induced IL 2 levels after radical lung cancer resection were higher than those without lung cancer. [Conclusion] The dysfunction of Th1/Th2 cell subsets in patients with lung cancer may be one of the mechanisms of lung cancer tissue growth and immune evasion in patients. The imbalance of Th1/Th2 subpopulations in lung cancer patients is at different stages and different pathological types of lung cancer. There was no difference between patients, and surgery could improve the dysfunctional status of Th1/Th2 subgroups in lung cancer patients.