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利用原代大鼠气管上皮（ＲＴＥ）细胞研究Ｎ－甲基－Ｎ′－硝基－Ｎ－亚硝基胍（ＭＮＮＧ）的致转化作用，并对转化细胞进行细胞遗传学研究．结果表明：增殖旺盛细胞（ＥＧＶ）集落是ＲＴＥ细胞体外转化最早期的特征，ＥＧＶ转化频率与ＭＮＮＧ剂量呈明显的剂量反应关系．从０．３ｍｇＬ－１ＭＮＮＧ剂量组分离到２个ＥＧＶ集落，经消化，传代形成了永生化的细胞系（ＲＴＥＳ１，ＲＴＥＳ２），ＲＴＥＳ１为多倍体核型，２号和７号染色体数目增加至４个且呈高频发生（１００％）；ＲＴＥＳ２呈二倍体核型．电镜结果证实转化细胞来自大鼠气管上皮组织．以上结果提示，原代ＲＴＥ细胞体外培养模型是研究致癌物定量及致癌机理的理想模型系统．
The primary rat tracheal epithelial (RTE) cells were used to study the transformation of N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) and the cytogenetic study of transformed cells. The results showed that proliferating cells (EGV) colonies were the earliest in vitro transformation of RTE cells, and the frequency of EGV transformation was significantly dose-dependent. Two EGV colonies were isolated from the 0.3 mg L-1 MNNG dose group and were digested and passaged to form immortalized cell lines (RTES1, RTES2). The RTES1 was polyploid karyotype and the number of chromosomes 2 and 7 increased to 4 And occurred in high frequency (100%); RTES2 diploid karyotype. Electron microscopy confirmed that the transformed cells came from the rat tracheal epithelium. The above results suggest that the primary culture model of RTE cells in vitro is an ideal model system to study carcinogen quantitative and carcinogenic mechanisms.