抗原:在感染人体巴西利什曼原虫原鞭毛体的非洲绿猴的单层肾脏Vero细胞中生长的原虫,经用15%兔血琼脂斜面培养使其大量繁殖后,浮悬在Eagle基础保种培养基中,并接种于组织培养,在35℃孵箱内培育6天后,倾去保种培养基以去掉细胞外的原虫,将感染原虫的细胞放入佛罗拿缓冲盐水(VBS)中,用0.02%EDTA处理,使感染原虫的细胞破裂,释出细胞里的无鞭毛原虫 Antigen: A protozoa growing in monolayer kidney Vero cells of African green monkey infected with Leishmania braziliensis promastigotes was multiplied by 15% rabbit blood agar slant culture and then suspended in Eagle’s basal Medium and inoculated into tissue culture. After incubating in a 35 ° C incubator for 6 days, the preservative medium was decanted to remove extracellular protozoa, and the cells infected with protozoa were placed in Florian Bucket Saline (VBS) Treatment with 0.02% EDTA ruptured the cells infected with the protozoa, releasing the flagellates