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植物名称:毛樱桃(Prunus tomentosa) 材料类别:茎尖及带腋芽茎段培养条件:基本培养基为L(Lepoivre),其成份(mg/1)NH_4NO_3,400;KNO_3,1800; Ca(NO_3)_2·4H_2O,1200;KH_2PO_4270; MgSO_4·7H_2O,360;MnSO_4·4H_2O,1.0;KI,0.08;其余同MS。诱导芽增殖而附加的激素为玉米素(ZT)和IBA,其浓度各为0.5,1.0。蔗糖 3％。诱导生根为 1/2L,附加NAA0.1～1.0,蔗糖 2％。琼脂均为 0.6％,温度25°±2℃,光强 1500～20001ux,16小时光照。生长与分化情况:接种10天左右,即可见到茎尖明显生长。不管外植体的初次培养还是以后的继代培养均以ZT1.0、IBA1.0mg/1效果较好,其增殖比率为1.1～3.0。适于生根的有效新梢为41～58％。将培养的1厘米以上的新梢转入生根培养基,
Plant Name: Prunus tomentosa Material Category: stem tip and axillary buds Stem culture conditions: the basic medium is L (Lepoivre), its composition (mg / 1) NH_4NO_3,400; KNO_3,1800; _2 · 4H_2O, 1200; KH_2PO_4270; MgSO_4 · 7H_2O, 360; MnSO_4 · 4H_2O, 1.0; KI, 0.08; Induced bud proliferation with additional hormones Zeatin (ZT) and IBA at concentrations of 0.5, 1.0 each. Sucrose 3%. Induced rooting was 1 / 2L, additional NAA0.1 ~ 1.0, sucrose 2%. Agar were 0.6%, temperature 25 ° ± 2 ℃, light intensity 1500 ~ 20001ux, 16 hours light. Growth and differentiation: Inoculation about 10 days, you can see the sharp growth of shoot tips. Regardless of explant primary culture or subsequent subculture are ZT1.0, IBA1.0mg / 1 better, the proliferation rate of 1.1 to 3.0. Effective shoots suitable for rooting 41 to 58%. Will be cultivated more than 1 cm shoots into rooting medium,