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目的：建立成熟稳定的异种肾移植（猪／人）间接体内模型，用以研究异种移植超急排斥反应的发生机制及对异种移植物所造成的损害。方法：猪肾２２只，为实验组；人肾８只，为对照组。利用体外灌注系统将人新鲜血经肝素抗凝、氧饱和后，３７℃条件下灌注离体肾脏。检测灌注前后血氧饱和度的变化和组织病理学改变。结果：两组肾脏灌注前、后的血氧饱和度变化无显著性差异。组织病理学检查发现：实验组的２２只猪肾在灌注开始后的５～７ｍｉｎ内即出现超急排斥反应征象；而对照组的８只人肾经人血灌注前后无明显变化。结论：本实验采用模拟人类生理条件下的体外灌注系统，建立了与实际临床应用最为接近的异种肾移植间接体内模型。此模型操作简便、结果稳定，可利用其对异种肾移植（猪／人）进行更广泛的深入研究。
Objective: To establish an indirect and in vivo model of mature and stable heterologous kidney transplantation (pig / human) to study the mechanism of xenotransplantation hyperacute rejection and its damage to xenografts. Methods: Twenty-two pigs were selected as experimental group and 8 as human control group. In vitro perfusion system will be the new blood of human anticoagulant heparin, oxygen saturation, 37 ℃ under the conditions of perfusion of isolated kidneys. Changes in blood oxygen saturation and histopathology before and after perfusion were measured. Results: There was no significant difference in oxygen saturation between the two groups before and after renal perfusion. Histopathological examination revealed that 22 piglets in the experimental group showed signs of hyperacute rejection within 5 to 7 minutes after the start of perfusion. In the control group, 8 piglets showed no significant changes before and after human blood perfusion. Conclusion: In this experiment, an in vitro model of in vitro renal allograft with the closest clinical application was established by simulating the in vitro perfusion system under human physiological conditions. This model is simple to operate and has stable results and allows for a more extensive and in-depth study of xenotransplantation (pig / human).