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目的了解引起无菌性脑膜炎暴发的Echo30病毒的基因特征,分析其遗传变异规律及进化来源。方法对2003年济南章丘市无菌性脑膜炎暴发中分离到的Echo330病毒进行了VP1区全基因序列测定,与国内外报道的Echo30病毒VP1区序列进行同源性比较,并通过构建进化树分析其遗传进化规律。结果济南章丘市Echo30分离株VP1区核苷酸同源性在98．9％～99．5％之间,与同年山东省泰安市Echo30分离株同源性最高(98．5％～99．0％),与浙江、江苏两省的Echo30分离株同源性在98．2％～98．9％,而与1997年法国分离株(89．1％～89 7％)和2001年台湾省分离株(87．4％～87．6％)的同源性相差较大,与原型株Bastianni株同源性最低,在82．4％～82．8％。进化树分析显示,中国大陆近几年无菌性脑膜炎Echo30分离株独处一支,呈单源性发生关系。结论2003年,Echo30病毒在山东省部分地区发生了一定程度的传播,并导致了无菌性脑膜炎的局部流行,进化树分析表明,中国大陆Echo30分离株与国外早期的分离株相比,其抗原性不完全相同,已形成新的基因亚型。
Objective To understand the genetic characteristics of Echo30 virus that causes aseptic meningitis outbreak and to analyze its genetic variation and its origin. Methods The complete VP1 gene sequence of Echo330 virus isolated from the outbreak of aseptic meningitis in Zhangqiu City of Jinan City in 2003 was determined. The homology was compared with the VP1 region of Echo30 virus reported in China and abroad. By constructing phylogenetic tree Analysis of the law of genetic evolution. Results The nucleotide homology of VP1 of Echo30 isolate in Zhangqiu city of Jinan was between 98.9% and 99.5%, which was the highest homology (98.5% -99%) to that of Echo30 isolate in Tai’an city of Shandong province in the same year. 0%), and isolates of Echo30 in Zhejiang and Jiangsu Provinces shared 98.2% -98.9% identity with those of French isolates (89.1% ~ 89.7%) in 1997 and Taiwan Province The homologies of isolates (87.4% ~ 87.6%) were quite different from those of the original strain, Bastianni, with the lowest homology of 82.4% ~ 82.8%. Phylogenetic tree analysis showed that in China in recent years, aseptic meningitis Echo30 isolates were single and showed a monogenic relationship. Conclusions In 2003, Echo30 virus spread to some extent in some areas of Shandong Province and led to the local epidemic of aseptic meningitis. Phylogenetic tree analysis showed that the Echo30 isolate of mainland China Antigenicity is not exactly the same, has formed a new genotype.