论文部分内容阅读
目的:观察淫羊藿苷对PC12细胞的毒性作用以及不同给药方法、给药剂量对氧糖剥夺PC12细胞活力的影响。方法:将不同浓度的淫羊藿苷与PC12细胞作用24 h后,用MTT法检测细胞活力。用预给药1 h,氧糖剥夺期间给药2 h,预给药1 h+氧糖剥夺期间给药2 h方法给药,细胞复氧复糖24 h后,用MTT法检测细胞活力,预给药1 h+氧糖剥夺期间给药2 h组在造模结束后用倒置显微镜观察细胞形态。结果:PC12细胞用10~(-8)~10~(-4)mol/L淫羊藿苷孵育24 h后,细胞活力与正常组比较均显著下降(P<0.01);10~(-8)~10~(-5)mol/L淫羊藿苷预给药1 h均使模型细胞的活力显著升高(P<0.01);10~(-8)~10~(-4)mol/L淫羊藿苷氧糖剥夺期间给药2 h均使模型细胞的活力显著下降(P<0.01);10~(-8)~10~(-5)mol/L预给药1 h+氧糖剥夺期间给药2 h均使模型细胞的活力显著升高(P<0.01),使模型细胞数量增加,细胞形态改善。结论:淫羊藿苷与细胞的作用时间不宜过长,预给药1h+氧糖剥夺期间给药2 h对氧糖剥夺-复氧PC12细胞能起到较好的保护作用。
OBJECTIVE: To observe the toxic effects of icariin on PC12 cells and the effects of different administration methods and dosages on the viability of PC12 cells. Methods: Icariin with different concentrations of PC12 cells after 24 h, MTT assay cell viability. Pretreatment with 1 h, Oxygen deprivation period of 2 h, pre-administration of 1 h + oxygen deprivation administered 2 h method of drug delivery, cell reoxygenation after 24 h, cell viability was detected by MTT, pre After 1 h of administration and 2 h of administration of oxygen deprivation, morphological changes of cells were observed by inverted microscope after modeling. Results: After incubated with 10 ~ (-8) ~ 10 ~ (-4) mol / L icariin for 24 h, the viability of PC12 cells decreased significantly compared with the normal group (P <0.01) ) 10 ~ (-5) mol / L icariin for 1 h significantly increased the viability of model cells (P <0.01) L and 2 h after oxycodone deprivation, the viability of model cells was significantly decreased (P <0.01), while the levels of 10 ~ (-8) -10 -5 mol / L pretreatment for 1 h + Administration of 2 h during deprivation significantly increased the viability of model cells (P <0.01), increased the number of model cells and improved the cell morphology. CONCLUSION: Icariin does not take too long to act on cells, and it can protect Oxygen-glucose-deprivation-reoxygenation PC12 cells in hypoxia-deprived and reoxygenated PC12 cells for 2 h after pretreatment of 1 h and oxygen-glucose deprivation.