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通过杂交育种技术对丹参的两个不同品种进行杂交,得到的子一代试管苗经同工酶电泳分析,根据亲本与子代之间农艺性状的差异,鉴定得h-1等几个杂交种株系。并进行了杂交株系田间农艺性状观察比较及根部药材主要有效化学成分的HPLC测定。对杂交种F1代大量扩繁后,采用秋水仙碱进行化学诱导异源多倍体,并鉴定所得诱导株系。试验结果表明秋水仙素浓度为(10~15)μg/g是诱导多倍体的最适宜浓度,并鉴定得到hz4-3、hz4-11等7个异源四倍体株系。为进一步选育异源多倍体优良品种奠定了基础。
Cross hybridization breeding was conducted on two different varieties of Salvia miltiorrhiza Bunge and the first generation of test-tube seedlings were analyzed by isozyme electrophoresis. According to the difference of agronomic characters between parents and offspring, several hybrid strains such as h-1 were identified system. The field agronomic traits of hybrid lines were observed and compared, and the main active chemical constituents in roots were determined by HPLC. After a large number of F1 hybrids were expanded, the colchicine was used to induce allopolyploid and the induced lines were identified. The results showed that colchicine concentration (10-15) μg / g was the most suitable concentration for inducing polyploidy, and 7 heterologous tetraploid strains such as hz4-3 and hz4-11 were identified. This laid the foundation for further breeding of elite polyploid varieties.