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策勒黑羊具有全年发情、多胎的特点,是新疆策勒县特有的优良绵羊品种。为研究与保护策勒黑羊遗传资源,为后续开展逆转录酶聚合酶链式反应(RT-PCR)、斑点杂交试验和构建c DNA文库试验等提供条件,试验采用经典异硫氰酸胍(Trizol)法提取策勒黑羊卵巢总RNA,通过核酸蛋白仪测定所提取RNA在不同波长下的吸光度值,计算RNA的提取量与纯度,并利用琼脂糖凝胶电泳显示提取结果和验证RNA的完整性。结果表明:采用Trizol法提取得到的策勒黑羊卵巢RNA的浓度、纯度与完整性均可达到后续试验的要求,可为后续策勒黑羊种质资源保护和利用等生物学试验的开展提供参考依据。
Cele black sheep with annual heat, multiple births, is a unique fine sheep in Cele County, Xinjiang. In order to study and protect the Celestial black sheep genetic resources, provide the conditions for the follow-up RT-PCR, dot blot hybridization and construction of c DNA library test. The experiment uses the classical guanidine isothiocyanate Trizol method was used to extract the total RNA from the ovaries of Celt black sheep. The absorbance values of the extracted RNA at different wavelengths were measured by a nucleic acid protein analyzer to calculate the amount and purity of the RNA extracted. The results were extracted and verified by agarose gel electrophoresis Integrity. The results showed that the concentration, purity and integrity of the RNA extracted from Cezer black sheep obtained by Trizol method could reach the requirements of the follow-up test, which could be used for the biological experiments such as the protection and utilization of germplasm resources in Ceil Black Sheep Reference.